Skip to main content
Kent Academic Repository

Array comparative genomic hybridisation on first polar bodies suggests that non-disjunction is not the predominant mechanism leading to aneuploidy in humans

Gabriel, A. S., Thornhill, Alan R., Ottolini, C. S., Gordon, Andrew, Brown, A. P. C., Taylor, J., Bennett, Katy, Handyside, Alan H, Griffin, Darren K. (2011) Array comparative genomic hybridisation on first polar bodies suggests that non-disjunction is not the predominant mechanism leading to aneuploidy in humans. Journal of Medical Genetics, 48 (7). pp. 433-437. ISSN 0022-2593. (doi:10.1136/jmg.2010.088070) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:31376)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1136/jmg.2010.088070

Abstract

Introduction. Aneuploidy (the presence of extra or missing chromosomes) arises primarily through chromosome segregation errors in the oocyte at meiosis I but the details of mechanism by which such errors occur in humans are the subject of some debate. It is generally believed that aneuploidy arises primarily as a result of segregation of a whole chromosome to the same pole as its homologue (non-disjunction). Nonetheless, classical cytogenetic studies suggest that this model does not fully account for the patterns observed in human oocytes. An alternative model (precocious separation of sister chromatids) has thus been proposed, but recurring criticism of this model purports that technical issues may have led to interpretation errors.

Materials and methods. Array comparative genomic hybridisation (aCGH) was used on 164 human first polar bodies to distinguish between whole chromosome (non-disjunction) and chromatid (precocious separation) errors.

Results. Single chromatid errors were over 11 times more common than whole chromosome errors, consistent with prior classical cytogenetic and fluorescence in situ hybridisation (FISH) studies.

Discussion. The received wisdom that non-disjunction is the primary mechanism leading to human aneuploidy should be reconsidered.

Item Type: Article
DOI/Identification number: 10.1136/jmg.2010.088070
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 08 Oct 2012 13:21 UTC
Last Modified: 16 Nov 2021 10:09 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/31376 (The current URI for this page, for reference purposes)

University of Kent Author Information

Handyside, Alan H.

Creator's ORCID:
CReDIT Contributor Roles:

Griffin, Darren K..

Creator's ORCID: https://orcid.org/0000-0001-7595-3226
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.