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Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of 1 integrin

Chen, Lixiang, Hughes, Richard A., Baines, Anthony J., Conboy, John G., Mohandas, Narla, An, Xiuli (2011) Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of 1 integrin. Journal of Cell Science, 124 (14). pp. 2478-2487. ISSN 0021-9533. (doi:10.1242/jcs.078170) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:31345)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1242/jcs.078170

Abstract

Protein 4.1R is a membrane-cytoskeleton adaptor protein that has diverse roles in controlling the cell surface expression and/or function of transmembrane proteins, and in organizing F-actin. 4.1R is expressed in keratinocytes, but its role in these cells has not been explored. Here, we have investigated the role of 4.1R in skin using 4.1R?/? mice. Cell adhesion, spreading, migration and motility were significantly impaired in 4.1R?/? keratinocytes, and 4.1R?/? mice exhibited defective epidermal wound healing. Cultured 4.1R?/? keratinocytes on fibronectin failed to form actin stress fibres and focal adhesions. Furthermore, in the absence of 4.1R, the surface expression, and consequently the activity of ?1 integrin were reduced. These data enabled the identification of a functional role for protein 4.1R in keratinocytes by modulating the surface expression of ?1 integrin, possibly through a direct association between 4.1R and ?1 integrin.

Item Type: Article
DOI/Identification number: 10.1242/jcs.078170
Uncontrolled keywords: 4.1R; ?1 integrin; Cell motility; Keratinocyte
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 08 Oct 2012 10:18 UTC
Last Modified: 16 Nov 2021 10:09 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/31345 (The current URI for this page, for reference purposes)

University of Kent Author Information

Baines, Anthony J..

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