Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses

Wright, Edward and Hayman, David T. S. and Vaughan, Aisling and Temperton, Nigel J. and Wood, James L. N. and Cunningham, Andrew A. and Suu-Ire, Richard and Weiss, Robin A. and Fooks, Anthony R. (2010) Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses. Virology, 408 (2). pp. 183-189. ISSN 0042-6822. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fruit bat, Eidolon helvum. Highly correlated titres were observed between single and multiplex PNAs using ptLBV and ptMOKV (r=0.97, p< 0.0001), validating its use for bat serosurveillance. Of the bat serum samples screened 56% neutralised ptLBV, 27% ptMOKV and 1% ptWCBV. Mean VNAb titres were 1:266, 1:35 and 1:7 against ptLBV, ptMOKV and ptWCBV respectively. The high seroprevalence estimates suggest that the infection rate of LBV in E. helvum remains high enough to persist in this species. This supports the hypothesis that LBV is endemic in Ghanaian E. helvum and we speculate that LBV may have co-evolved with African megachiroptera.

Item Type: Article
Uncontrolled keywords: Africa, Antibody, Bat, Ghana, Lagos bat virus, Lyssavirus, Neutralisation, Pseudotype, Rabies virus, Serology, animal cell, antibody titer, article, bat, cell strain BHK, cell strain HEK293, controlled study, endemic disease, female, Ghana, human, human cell, infection rate, male, nonhuman, nucleotide sequence, persistent infection, priority journal, pseudotyping, Rabies virus, serology, seroprevalence, species difference, validation process, virus carrier, virus infection, virus load, virus neutralization, Animals, Antibodies, Viral, Biological Evolution, Chiroptera, Communicable Diseases, Emerging, Disease Reservoirs, Female, Ghana, HEK293 Cells, Humans, Lyssavirus, Male, Neutralization Tests, Rhabdoviridae Infections, Species Specificity, Eidolon helvum, Lagos bat virus, Lyssavirus, Megachiroptera, Mokola virus, Rabies virus, West Caucasian Bat virus
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 09:51
Last Modified: 10 Jun 2014 08:14
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