A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins

Corti, Davide, Voss, Jarrod, Gamblin, Steven J., Codoni, Giosiana, Macagno, Annalisa, Jarrossay, David, Vachieri, Sebastien G., Pinna, Debora, Minola, Andrea, Vanzetta, Fabrizia, and others. (2011) A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science, 333 (6044). pp. 850-856. ISSN 0036-8075. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

Item Type: Article
Uncontrolled keywords: Influenza virus hemagglutinin, monoclonal antibody F16, monoclonal antibody F16v3, neutralizing antibody, unclassified drug, antibody, biochemistry, crystallography, cytology, disease treatment, influenza, neutralization, plasma, protein, virus, X-ray spectroscopy, animal experiment, animal model, antibody isolation, article, cell culture, controlled study, drug design, ferret, influenza A, Influenza virus A, mouse, nonhuman, passive immunization, plasma cell, priority journal, X ray crystallography, Animals, Antibodies, Neutralizing, Antibodies, Viral, Antibody Specificity, Antigens, Viral, Cells, Cultured, Cross Reactions, Crystallography, X-Ray, Epitopes, Ferrets, Glycosylation, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Hydrophobic and Hydrophilic Interactions, Immunization, Passive, Immunoglobulin Variable Region, Influenza A virus, Influenza A Virus, H1N1 Subtype, Influenza B virus, Influenza, Human, Mice, Models, Molecular, Molecular Sequence Data, Orthomyxoviridae Infections, Plasma Cells, Protein Multimerization, Protein Structure, Secondary, Influenza A virus, Mus, Mustela
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 02 Aug 2012 06:56 UTC
Last Modified: 29 May 2019 08:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/29397 (The current URI for this page, for reference purposes)
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