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A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application

Grotzkyj-Giorgi, Margherita, Howland, Kevin, Martin, Colin R., Bonner, Adrian B. (2012) A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application. Scientific World Journal, 2012 . pp. 1-8. ISSN 1537-744X. (doi:10.1100/2012/359721) (KAR id:29204)

Abstract

An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.

Item Type: Article
DOI/Identification number: 10.1100/2012/359721
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 27 Mar 2012 13:18 UTC
Last Modified: 05 Nov 2024 10:10 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/29204 (The current URI for this page, for reference purposes)

University of Kent Author Information

Grotzkyj-Giorgi, Margherita.

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Howland, Kevin.

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Bonner, Adrian B..

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