Reversible movement of switch 1 loop of myosin determines actin interaction

Kintses, Bálint and Gyimesi, Máté and Pearson, David S. and Geeves, Michael A. and Zeng, Wei and Bagshaw, Clive R and Malnasi-Csizmadia, András (2007) Reversible movement of switch 1 loop of myosin determines actin interaction. EMBO Journal, 26 (1). pp. 265-274. ISSN 0261-4189 . (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://www.nature.com/emboj/journal/v26/n1/pdf/760...

Abstract

The conserved switch 1 loop of P-loop NTPases is implicated as a central element that transmits information between the nucleotide-binding pocket and the binding site of the partner proteins. Recent structural studies have identified two states of switch 1 in G-proteins and myosin, but their role in the transduction mechanism has yet to be clarified. Single tryptophan residues were introduced into the switch 1 region of myosin II motor domain and studied by rapid reaction methods. We found that in the presence of MgADP, two states of switch 1 exist in dynamic equilibrium. Actin binding shifts the equilibrium towards one of the MgADP states, whereas ATP strongly favors the other. In the light of electron cryo-microscopic and X-ray crystallographic results, these findings lead to a specific structural model in which the equilibrium constant between the two states of switch 1 is coupled to the strength of the actin-myosin interaction. This has implications for the enzymatic mechanism of G-proteins and possibly P-loop NTPases in general

Item Type: Article
Uncontrolled keywords: actomyosin interaction; G-proteins; Mg-binding; nucleotide binding; tryptophan fluorescence
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Maureen Cook
Date Deposited: 25 Apr 2008 09:51
Last Modified: 16 Jul 2014 11:01
Resource URI: https://kar.kent.ac.uk/id/eprint/2838 (The current URI for this page, for reference purposes)
  • Depositors only (login required):