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Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast

Merritt, Gloria H., Naemi, Wesley R., Mugnier, Pierre M., Webb, Helen M., Tuite, Mick F., von der Haar, Tobias (2010) Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast. Nucleic acids research, 38 (16). pp. 5479-5492. ISSN 1362-4962. (doi:10.1093/nar/gkq338) (KAR id:25568)

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Abstract

Translation termination in eukaryotes typically requires the decoding of one of three stop codons UAA, UAG or UGA by the eukaryotic release factor eRF1. The molecular mechanisms that allow eRF1 to decode either A or G in the second nucleotide, but to exclude UGG as a stop codon, are currently not well understood. Several models of stop codon recognition have been developed on the basis of evidence from mutagenesis studies, as well as studies on the evolutionary sequence conservation of eRF1. We show here that point mutants of Saccharomyces cerevisiae eRF1 display significant variability in their stop codon read-through phenotypes depending on the background genotype of the strain used, and that evolutionary conservation of amino acids in eRF1 is only a poor indicator of the functional importance of individual residues in translation termination. We further show that many phenotypes associated with eRF1 mutants are quantitatively unlinked with translation termination defects, suggesting that the evolutionary history of eRF1 was shaped by a complex set of molecular functions in addition to translation termination. We reassess current models of stop-codon recognition by eRF1 in the light of these new data.

Item Type: Article
DOI/Identification number: 10.1093/nar/gkq338
Subjects: Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Sciences > School of Biosciences
Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Tobias von der Haar
Date Deposited: 24 Sep 2010 12:19 UTC
Last Modified: 06 May 2020 03:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/25568 (The current URI for this page, for reference purposes)
Tuite, Mick F.: https://orcid.org/0000-0002-5214-540X
von der Haar, Tobias: https://orcid.org/0000-0002-6031-9254
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