Hayes, M.V., Tuite, Mick F. (1992) ELONGATION-FACTOR-3 (EF-3) FROM CANDIDA-ALBICANS SHOWS BOTH STRUCTURAL AND FUNCTIONAL SIMILARITY TO EF-3 FROM SACCHAROMYCES-CEREVISIAE. Molecular Microbiology, 6 (8). pp. 1025-1033. ISSN 0950-382X. (doi:10.1111/j.1365-2958.1992.tb02168.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:23269)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb02168... |
Abstract
As with many other fungi, including the budding yeast Saccharomyces cerevisiae, the dimorphic fungus Candida albicans encodes the novel translation factor, elongation factor 3 (EF-3). Using a rapid affinity chromatography protocol, EF-3 was purified to homogeneity from C. albicans and shown to have an apparent molecular mass of 128kDa. A polyclonal antibody raised against C. albicans EF-3 also showed cross-reactivity with EF-3 from S. cerevisiae. Similarly, the S. cerevisiae TEF3 gene (encoding EF-3) showed cross-hybridization with genomic DNA from C. albicans in Southern hybridization analysis, demonstrating the existence of a single gene closely related to TEF3 in the C. albicans genome. This gene was cloned by using a 0.7kb polymerase chain reaction-amplified DNA fragment to screen a C. albicans gene library. DNA sequence analysis of 200 bp of the cloned fragment demonstrated an open reading frame showing 51% predicted amino acid identity between the putative C. albicans EF-3 gene and its S. cerevisiae counterpart over the encoded 65-amino-acid stretch. That the cloned C. albicans sequence did indeed encode EF-3 was confirmed by demonstrating its ability to rescue an otherwise non-viable S. cerevisiae tef3:HIS3 null mutant. Thus EF-3 from C. albicans shows both structural and functional similarlity to EF-3 from S. cerevisiae.
Item Type: | Article |
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DOI/Identification number: | 10.1111/j.1365-2958.1992.tb02168.x |
Subjects: |
Q Science > QP Physiology (Living systems) > QP517 Biochemistry Q Science > QR Microbiology Q Science > QP Physiology (Living systems) > QP506 Molecular biology |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | A. Xie |
Date Deposited: | 01 Nov 2009 00:37 UTC |
Last Modified: | 16 Nov 2021 10:01 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/23269 (The current URI for this page, for reference purposes) |
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