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The Number and Transmission of [PSI+] Prion Seeds (Propagons) in the Yeast Saccharomyces Cerevisiae

Byrne, Lee J., Cole, Diana J., Cox, Brian S., Ridout, Martin S., Morgan, Byron J. T., Tuite, Mick F. (2009) The Number and Transmission of [PSI+] Prion Seeds (Propagons) in the Yeast Saccharomyces Cerevisiae. PLoS ONE, Online . ISSN eISSN-1932-6203. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:23092)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://www.plosone.org/article/info%3Adoi%2F10.137...

Abstract

Abstract Top

Yeast (Saccharomyces cerevisiae) prions are efficiently propagated and the on-going generation and transmission of prion seeds (propagons) to daughter cells during cell division ensures a high degree of mitotic stability. The reversible inhibition of the molecular chaperone Hsp104p by guanidine hydrochloride (GdnHCl) results in cell division-dependent elimination of yeast prions due to a block in propagon generation and the subsequent dilution out of propagons by cell division.

Principal Findings

While propagon generation is an ATP-driven process, the partition of propagons to daughter cells occurs by passive transfer via the distribution of cytoplasm. Furthermore, our new estimates of n0, the number of propagons per cell (500–1000), are some five times higher than our previous estimates and this has important implications for our understanding of the inheritance of the [PSI+] and the spontaneous formation of prion-free cells.

Item Type: Article
Additional information: Research Article
Subjects: Q Science > QA Mathematics (inc Computing science) > QA276 Mathematical statistics
Q Science > QK Botany
Q Science > QR Microbiology
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Sciences > School of Mathematics Statistics and Actuarial Science > Statistics
Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Diana Cole
Date Deposited: 26 Oct 2009 12:52 UTC
Last Modified: 06 May 2020 03:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/23092 (The current URI for this page, for reference purposes)
Cole, Diana J.: https://orcid.org/0000-0002-8109-4832
Tuite, Mick F.: https://orcid.org/0000-0002-5214-540X
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