Expression of the rabbit intestinal n2 na+ nucleoside transporter in xenopus-laevis oocytes

Jarvis, Simon M. and Griffith, Douglas A. (1991) Expression of the rabbit intestinal n2 na+ nucleoside transporter in xenopus-laevis oocytes. Biochemical Journal, 278 . pp. 605-607. ISSN 0264-6021. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

Polyadenylated [poly(A)+] mRNA isolated from rabbit small-intestinal mucosa was injected into Xenopus laevis oocytes, and expression of the N2 Na+/nucleoside co-transporter was assayed by measuring Na+-dependent thymidine uptake. Expression of Na+-dependent thymidine uptake steadily increased after mRNA injection and was on average increased 11-fold by day 6 over background. Na+-dependent thymidine uptake was saturable (apparent K(m) approximately 30-mu-M at 22-degrees-C) and inhibited by uridine and cytidine, but not by guanosine and inosine. These properties of the expressed thymidine transport strongly suggest that the epithelial N2 Na+/nucleoside co-transporter can be expressed in X. laevis oocytes.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 03 Nov 2009 17:57
Last Modified: 20 Jun 2014 14:04
Resource URI: https://kar.kent.ac.uk/id/eprint/22992 (The current URI for this page, for reference purposes)
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