Skip to main content
Kent Academic Repository

Expression of the rabbit intestinal n2 na+ nucleoside transporter in xenopus-laevis oocytes

Jarvis, Simon M., Griffith, Douglas A. (1991) Expression of the rabbit intestinal n2 na+ nucleoside transporter in xenopus-laevis oocytes. Biochemical Journal, 278 . pp. 605-607. ISSN 0264-6021. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:22992)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.

Abstract

Polyadenylated [poly(A)+] mRNA isolated from rabbit small-intestinal mucosa was injected into Xenopus laevis oocytes, and expression of the N2 Na+/nucleoside co-transporter was assayed by measuring Na+-dependent thymidine uptake. Expression of Na+-dependent thymidine uptake steadily increased after mRNA injection and was on average increased 11-fold by day 6 over background. Na+-dependent thymidine uptake was saturable (apparent K(m) approximately 30-mu-M at 22-degrees-C) and inhibited by uridine and cytidine, but not by guanosine and inosine. These properties of the expressed thymidine transport strongly suggest that the epithelial N2 Na+/nucleoside co-transporter can be expressed in X. laevis oocytes.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Q Science > QP Physiology (Living systems) > QP506 Molecular biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: O.O. Odanye
Date Deposited: 03 Nov 2009 17:57 UTC
Last Modified: 16 Nov 2021 10:01 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/22992 (The current URI for this page, for reference purposes)

University of Kent Author Information

Jarvis, Simon M..

Creator's ORCID:
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.