Multiple Sodium-Dependent Nucleoside Transport-Systems in Bovine Renal Brush-Border Membrane-Vesicles

Williams, T.C. and Jarvis, Simon M. (1991) Multiple Sodium-Dependent Nucleoside Transport-Systems in Bovine Renal Brush-Border Membrane-Vesicles. Biochemical Journal, 274 (1). pp. 27-33. ISSN 0264-6021. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)

Abstract

Na+-dependent nucleoside transport was examined in bovine renal brush-border membrane vesicles. Two separate Na+/nucleoside cotransporters were shown to be present: (1) a system specific for purine nucleosides and uridine, designated as the N1 carrier, and (2) an Na+-dependent nucleoside transporter that accepts pyrimidine nucleosides, adenosine and analogues of adenosine, designated as the N2 system. Both systems exhibit a high affinity for nucleosides (apparent K(m) values approximately 10-mu-M), are insensitive to inhibition by facilitated-diffusion nucleoside transport inhibitors, are rheogenic and exhibit a high specificity for Na+. Na+ increases the affinity of the influx of guanosine and thymidine, nucleosides that serve as model permeants for the N1 and N2 nucleoside transporters respectively. The Na+/nucleoside coupling stoichiometry is consistent with 1:1 for both carriers.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 29 Sep 2009 07:03
Last Modified: 20 Jun 2014 14:04
Resource URI: https://kar.kent.ac.uk/id/eprint/22800 (The current URI for this page, for reference purposes)
  • Depositors only (login required):