The Effects of 5'-Capping, 3'-Polyadenylation and Leader Composition Upon the Translation and Stability of Messenger-Rna in a Cell-Free-Extract Derived from the Yeast Saccharomyces-Cerevisiae

Gerstel, Birgit and Tuite, Mick F. and McCarthy, John E. G. (1992) The Effects of 5'-Capping, 3'-Polyadenylation and Leader Composition Upon the Translation and Stability of Messenger-Rna in a Cell-Free-Extract Derived from the Yeast Saccharomyces-Cerevisiae. Molecular Microbiology, 6 (16). pp. 2339-2348. ISSN 0950-382X. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1111/j.1365-2958.1992.tb01409...

Abstract

A new modular expression system was developed to direct the in vitro synthesis of defined transcripts that were used as templates for translation in yeast cell-free extracts. The system was used to examine the influence of 5'-capping, 3'-polyadenylation and leader sequence upon the translation and stability of the synthetic Tn9 cat (chloramphenicol acetyl transferase), yeast PGK (phosphoglycerate kinase) and yeast HSP26 (heat-shock protein 26) mRNAs. The addition of a methylated cap (m7Gppp) or of a poly(A) tall enhanced translation and stabilized the mRNA. The dependence of translation upon capping was reduced in the presence of the HSP26 leader sequence. This may indicate the existence of a translational mechanism that enhances cap-independent translation. The enhancement of the translation and stability of mRNA was relatively insensitive to changes in the position of the poly(A) tail relative to the reading frame.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 05 Sep 2009 12:24
Last Modified: 10 Jun 2014 13:05
Resource URI: https://kar.kent.ac.uk/id/eprint/22574 (The current URI for this page, for reference purposes)
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