Covalent Immobilization Laccase on Activated Carbon for Phenolic Efflunce Treatment

Davis, S. and Burns, Richard G. (1992) Covalent Immobilization Laccase on Activated Carbon for Phenolic Efflunce Treatment. Applied Microbiology and Biotechnology, 37 (4). pp. 474-479. ISSN 0175-7598. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Laccase was covalently immobilised to activated carbon using four derivatisation methods. The highest bound activity was obtained using diimide coupling of laccase to carboxyl groups on the carbon. The maximum bound activity was reached at 11.5 mg laccase/g carbon; the maximum protein bound was 42.85 mg/g carbon. The carbon-immobilised laccase (CIL) was stable at pH values from 4.0 to 9.0. CIL stored at 4-degrees-C lost 38 +/- 5 % activity in the first 4 days, then a further 22 +/- 5 % in 126 days. CIL showed increased stability to low pH although the pH optimum was unchanged. The activation energy of CIL was lower than soluble laccase. Oxidation of 2,6-dimethoxyphenol (DMP) by CIL in a packed-bed system was only 30 +/- 10 % of that in a fluidised bed system. Of the initial activity 10-30 % was retained after oxidation of seven batches of DMP. CIL removed colour from two industrial effluents. Colour was removed from pulp mill bleach plant effluent at 115 colour units per enzyme unit per hour and the removal rate increased with increasing effluent concentration.

Item Type: Article
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 10 Oct 2009 12:27
Last Modified: 13 May 2014 13:24
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