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The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter

Allen, Marcus J., Murphey, R.K. (2007) The chemical component of the mixed GF-TTMn synapse in Drosophila melanogaster uses acetylcholine as its neurotransmitter. European Journal of Neuroscience, 26 (2). pp. 439-445. ISSN 0953-816X. (doi:10.1111/j.1460-9568.2007.05686.x) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:2090)

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The largest central synapse in adult Drosophila is a mixed electro-chemical synapse whose gap junctions require the product of the shaking-B (shak-B) gene. Shak-B-2 mutant flies lack gap junctions at this synapse, which is between the giant fibre (GF) and the tergotrochanteral motor neuron (TTMn), but it still exhibits a long latency response upon GF stimulation. We have targeted the expression of the light chain of tetanus toxin to the GF, to block chemical transmission, in shak-B-2 flies. The long latency response in the tergotrochanteral muscle (TTM) was abolished indicating that the chemical component of the synapse mediates this response. Attenuation of GAL4-mediated labelling by a cha-GAL80 transgene, reveals the GF to be cholinergic. We have used a temperature-sensitive allele of the choline acetyltransferase gene (cha(ts2)) to block cholinergic synapses in adult flies and this also abolished the long latency response in shak-B-2 flies. Taken together the data provide evidence that both components of this mixed synapse are functional and that the chemical neurotransmitter between the GF and the TTMn is acetylcholine. Our findings show that the two components of this synapse can be separated to allow further studies into the mechanisms by which mixed synapses are built and function.

Item Type: Article
DOI/Identification number: 10.1111/j.1460-9568.2007.05686.x
Uncontrolled keywords: giant fibre; innexins; neuron; neurotransmitter; tetanus toxin
Subjects: R Medicine > RC Internal medicine > RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Stephen Holland
Date Deposited: 19 Dec 2007 19:27 UTC
Last Modified: 16 Nov 2021 09:40 UTC
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