Effect of temperature on protein conformation and activity during ultrafiltration

Campbell, Moray J. and Walter, Robert P. and McLoughlin, Robert and Knowles, Christopher J. (1993) Effect of temperature on protein conformation and activity during ultrafiltration. Journal of Membrane Science, 78 (1-2). pp. 35-43. ISSN 0376-7388. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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One of the advantages of ultrafiltration for the bioseparation of proteins is the comparatively mild conditions used. A key operational parameter in such a system is the temperature utilised for the process. There are conflicting demands when selecting an operating temperature between the fluid dynamics and the biochemical properties of the system. An analysis of this conflict, in order to establish the optimal temperature, involved a model crossflow filtration system, where shear rate, volume of protein containing solution and protein concentration can be kept constant and only the temperature varied. In such studies denaturation of the permeate was observed whereas the processing efficiency did not appear to have a temperature optimum.

Item Type: Article
Subjects: T Technology > TP Chemical technology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: R.F. Xu
Date Deposited: 06 Oct 2009 11:15
Last Modified: 18 Jun 2014 13:49
Resource URI: https://kar.kent.ac.uk/id/eprint/20818 (The current URI for this page, for reference purposes)
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