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Glycosylation of Recombination Proteins - Problems and Prospects

Jenkins, Nigel, Curling, Elizabeth M. (1994) Glycosylation of Recombination Proteins - Problems and Prospects. Enzyme and Microbial Technology, 16 (5). pp. 354-364. ISSN 0141-0229. (doi:10.1016/0141-0229(94)90149-X) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:20273)

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Glycosylation, the addition of sugar residues to a peptide backbone, is the most extensive posttranslational modifica- tion made to proteins by eukaryotic cells. The majority of recombinant proteins manufactured for human therapy are glycoproteins derived from animal cells, and it is essential to fully characterize and, if possible, control the glycosylation profile of these products. Indeed, the Food and Drug Ad- ministration (FDA) in the United States and the Commit- tee for Proprietary Medical Productions (CPMP) of the European Community are demanding increasingly sophis- ticated carbohydrate analysis on all new glycoproteins des- tined for human therapy.

A complete survey of protein glycosylation is beyond the scope of this article, and the reader is referred to the excel- lent reviews recently published on glycoprotein biosyn- thesis, 2-7 regulation of biosynthetic pathways, 1,8-13 biolog- ical functions of glycan structures, 1.14-19 and glycoprotein analysis. 2°-29 This review will focus on the aspects of glyco- protein research that are of direct relevance to the biotech- nology industry, i.e., the reasons and methods for defining the glycosylation patterns of recombinant glycoproteins

Item Type: Article
DOI/Identification number: 10.1016/0141-0229(94)90149-X
Uncontrolled keywords: Glycosylation, biosynthetic pathways; enzymes involved; biological significance; commercial implications; pharmacokinetics; antigenicity; solubility; Oligosaccharides; Glycosyltransferases; cell distribution
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: P. Ogbuji
Date Deposited: 25 Jun 2009 11:29 UTC
Last Modified: 16 Nov 2021 09:58 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Curling, Elizabeth M..

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