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Expression of Recombinant Receptors in Insect Cells

Rooney, Barrie C. and Strange, Philip G. and Woodcock, Christine (1994) Expression of Recombinant Receptors in Insect Cells. In: Kobayashi, T. and Kitagawa, Y. and Okumura, K., eds. Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93 . Kluwer Academic, Dordrecht, Netherlands, pp. 35-40. ISBN 978-0-7923-3156-8. (doi:10.1007/978-94-011-0848-5_5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:20005)

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Membrane bound receptors are key molecules in intracellular communication systems but structure function studies are often hampered by low level of native expression of these proteins. An essential feature of any recombinant system used to over express receptors is the authenticity of the recombinant product. The Baculovirus/insect cell expression system BEVS has been widely used to express many mammalian proteins as it has been shown to carry out many eukaryotic post-translational modifications. This paper will describe its use to over express members of the single transmembrane domain, tyrosine kinase receptor family (PDGF-receptors), and members of the G-protein linked, seven transmembrane domain family (Dopamine receptors). In particular production and activity studies on full length and truncated forms of the PDGF-receptors and comparisons made with mammalian expressed constructs will be discussed. A full pharmacological profile of recombinant dopamine receptors will be presented and the significance of variations between sub-types will be discussed in the context of possible physiological significance.

Item Type: Book section
DOI/Identification number: 10.1007/978-94-011-0848-5_5
Uncontrolled keywords: Dopamine Receptor; Insect Cell; Chinese Hamster Ovary Cell; Truncated Form; PDGF Receptor
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: P. Ogbuji
Date Deposited: 19 Jun 2009 14:04 UTC
Last Modified: 16 Nov 2021 09:58 UTC
Resource URI: (The current URI for this page, for reference purposes)

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Rooney, Barrie C..

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