Mammalian-cell damage in a novel membrane bioreactor

Millward, H.R. and Bellhouse, B.J. and Nicholson, Andrea M. and Beeton, S. and Jenkins, Nigel and Knowles, Christopher J. (1994) Mammalian-cell damage in a novel membrane bioreactor. Biotechnology and Bioengineering, 43 (9). pp. 899-906. ISSN 0006-3592. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The experimental study has assessed a novel membrane bioreactor for mammalian cell culture. In the absence of a gas phase, the key features of cell damage associated with laminar and turbulent flow have been identified. The bioreactor employs a dimpled membrane in order to enhance transverse mixing in a narrow channel, but a fall in viable cell density has been observed at Reynolds numbers above Re = 83. In the laminar flow regime wall shear is the critical mechanism and an accurate calculation of shear rate in a complex channel has been achieved using the Reynolds analogy. Flow generating a wall shear rate in excess of 3000 s(-1) has been shown to cause damage. Power dissipation measurements have been used to distinguish between laminar and turbulent flow and also to predict Kolmogorov eddy lengths. An additional turbulent bulk stress damage mechanism at higher Reynolds numbers (Re > 250) results in a very rapid fall in viable cell density.

Item Type: Article
Uncontrolled keywords: membrane bioreactor; mammalian cell damage; critical shear rate; power dissipation
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 06 Jul 2009 19:55
Last Modified: 15 Jul 2014 14:04
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