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Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms

Barros, L.F., Yudilevich, D.L., Jarvis, Simon M., Beaumont, N., Young, J.D., Baldwin, S.A. (1995) Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms. Pflügers Archiv, 429 (3). pp. 394-399. ISSN 0031-6768. (doi:10.1007/BF00374155) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19688)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/BF00374155

Abstract

Polyclonal antibodies raised against the human erythrocyte nucleoside transporter were used to investigate the distribution of the nucleoside transporters in the placenta. Immunoblots of brush-border membranes isolated from the human syncytiotrophoblast revealed a cross-reactive species that co-migrated with the erythrocyte nucleoside transporter as a broad band of apparent M(r) 55,000. In contrast, no labelling was detected in basal membranes containing a similar number of equilibrative nucleoside transporters as assessed by nitrobenzylthioinosine (NBMPR)-binding. The absence of cross-reactive epitopes in basal membranes and their presence in brush-border membranes was confirmed by confocal immunofluorescence microscopy. The results suggest that at least two isoforms of the NBMPR-sensitive nucleoside transporter are present in the human placenta. The lumenal surfaces of fetal capillaries, small placental vessels and umbilical vein ware also strongly labelled by the antibody, a finding that suggests that the high fetal-placental adenosine uptake previously reported is due to endothelial transporters.

Item Type: Article
DOI/Identification number: 10.1007/BF00374155
Uncontrolled keywords: NUCLEOSIDE TRANSPORT; NITROBENZYLTHIOINOSINE (NBMPR); MICROVILLOUS AND BASAL MEMBRANE VESICLES; PLACENTA (HUMAN)
Subjects: B Philosophy. Psychology. Religion > B Philosophy (General)
Divisions: Faculties > Social Sciences > School of Psychology
Depositing User: P. Ogbuji
Date Deposited: 08 Jun 2009 16:23 UTC
Last Modified: 28 May 2019 13:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19688 (The current URI for this page, for reference purposes)
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