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Production and post-translational modification of tPA in Namalwa cells

Khan, Mohsan W. and Musgrave, S.W. and Jenkins, Nigel (1995) Production and post-translational modification of tPA in Namalwa cells. In: Beuvery, E.C. and Griffiths, J.B. and Zeijlemaker, W.P., eds. Animal Cell Technology: Developments Towards the 21st Century. Kluwer Academic, Dordrecht, Netherlands, pp. 397-401. ISBN 0-7923-3736-0. E-ISBN 978-94-011-0437-1. (doi:10.1007/978-94-011-0437-1_63) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1007/978-94-011-0437-1_63

Abstract

N-linked glycosylation is a major post-translational event having profound influence on the quality of recombinant proteins destined for therapy and hence it is extensively studied [1]. Glycosylation is affected by the type of host cell, environmental stimuli, and protein structure [2]. This study will investigate the first two factors by characterising a model protein: tissue plasminogen activator (tPA) expressed in Chinese hamster ovary (CHO) and a human lymphoblastoid cell line (Namalwa). tPA is a fibrin-specific serine protease of plasminogen involved in the dissolution of vascular fibrin clots in vivo. It exists as a mixture of single-chain and two-chain (A and B) glycoproteins (66-72kDa) with four potential N-linked glycosylation sites at Asn117 (invariably high mannose type) Asn184 (variably occupied complex type) Asn218 (never glycosylated) and Asn448 (invariably complex type) and an O-linked fucose occurs at Thr61. Type I tPA has three sites occupied with N-linked oligosaccharides and type II has only two sites occupied [3].

Item Type: Book section
DOI/Identification number: 10.1007/978-94-011-0437-1_63
Uncontrolled keywords: Chinese Hamster Ovary; Oligosaccharide Structure; Glycosylation Pathway; High Mannose Type; Namalwa Cell
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 28 May 2009 17:47 UTC
Last Modified: 19 Jun 2019 15:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19647 (The current URI for this page, for reference purposes)
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