Greenall, Carole and Jenkins, Nigel and Tuite, Mick F. and Robinson, Colin and Cook, David and Freedman, Robert B. (1995) Cell engineering to optimise protein secretion: Analysis of components of the secretory system. In: Beuvery, E.C. and Griffiths, J.B. and Zeijlemaker, W.P., eds. Animal Cell Technology: Developments Towards the 21st Century. Kluwer Academic, pp. 27-31. ISBN 978-94-010-4195-9. E-ISBN 978-94-011-0437-1. (doi:10.1007/978-94-011-0437-1_5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19570)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1007/978-94-011-0437-1_5 |
Abstract
A series of recombinant clones, derived from the mouse NS/0 cell line, have been studied. These three clones secrete varying high levels of h1B4, a humanised monoclonal antibody. The secretion levels of protein have been measured by ELISA and the specific secretion rates calculated. These clones, and a series of wild-type cells have been analysed by western blotting using antibodies against various chaperone proteins and components of the protein secretory system. The activity of protein disulphide isomerase (PDI), the enzyme which catalyses protein folding, has been measured. The three cell lines showed no significant differences in PDI activity or levels of PDI, BiP, and ribophorin II. Further studies of the clones will be aimed at identifying possible rate-or yield-limiting steps in protein secretion.
Item Type: | Book section |
---|---|
DOI/Identification number: | 10.1007/978-94-011-0437-1_5 |
Uncontrolled keywords: | Protein Secretion; Recombinant Clone; Secretion Level; Protein Disulphide Isomerase; Cell Engineer |
Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | P. Ogbuji |
Date Deposited: | 01 Jun 2009 08:46 UTC |
Last Modified: | 16 Nov 2021 09:57 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/19570 (The current URI for this page, for reference purposes) |
- Export to:
- RefWorks
- EPrints3 XML
- BibTeX
- CSV
- Depositors only (login required):