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Cell engineering to optimise protein secretion: Analysis of components of the secretory system

Greenall, Carole and Jenkins, Nigel and Tuite, Mick F. and Robinson, Colin and Cook, David and Freedman, Robert B. (1995) Cell engineering to optimise protein secretion: Analysis of components of the secretory system. In: Beuvery, E.C. and Griffiths, J.B. and Zeijlemaker, W.P., eds. Animal Cell Technology: Developments Towards the 21st Century. Kluwer Academic, pp. 27-31. ISBN 978-94-010-4195-9. E-ISBN 978-94-011-0437-1. (doi:10.1007/978-94-011-0437-1_5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:19570)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/978-94-011-0437-1_5

Abstract

A series of recombinant clones, derived from the mouse NS/0 cell line, have been studied. These three clones secrete varying high levels of h1B4, a humanised monoclonal antibody. The secretion levels of protein have been measured by ELISA and the specific secretion rates calculated. These clones, and a series of wild-type cells have been analysed by western blotting using antibodies against various chaperone proteins and components of the protein secretory system. The activity of protein disulphide isomerase (PDI), the enzyme which catalyses protein folding, has been measured. The three cell lines showed no significant differences in PDI activity or levels of PDI, BiP, and ribophorin II. Further studies of the clones will be aimed at identifying possible rate-or yield-limiting steps in protein secretion.

Item Type: Book section
DOI/Identification number: 10.1007/978-94-011-0437-1_5
Uncontrolled keywords: Protein Secretion; Recombinant Clone; Secretion Level; Protein Disulphide Isomerase; Cell Engineer
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 01 Jun 2009 08:46 UTC
Last Modified: 06 May 2020 03:04 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/19570 (The current URI for this page, for reference purposes)
Tuite, Mick F.: https://orcid.org/0000-0002-5214-540X
Robinson, Colin: https://orcid.org/0000-0002-1739-032X
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