Construction, purification and immunogenicity of antigen-antibody-LTB complexes

Green, E.A. and Botting, Catherine and Webb, Helen M. and Hirst, Timothy R. and Randall, Richard Edward (1996) Construction, purification and immunogenicity of antigen-antibody-LTB complexes. Vaccine, 14 (10). pp. 949-958. ISSN 0264-410X. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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An oligonucleotide, encoding a short epitope peptide tag, termed Pk, was inserted at the 3'-end of the gene coding B-subunit of Escherichia coli heat-labile enterotoxin (LTB). The presence of the Pk epitope on LTB-Pk was used to construct novel macromolecular assemblies comprising LTB-Pk, an anti-Pk mAb, (mAb SV5-P-k) and Pk-linked recombinant SIV proteins. The 1:1:1 stoichiometry of such complexes was ensured by binding LTB-Pk to one arm of mAb SV5-P-k and an SIV-Pk antigen to the other arm of the antibody. Such SIV-mAb-LTB macromolecular complexes bound to GM1-ganglioside in vitro, and when immunized systemically into mice were highly immunogenic, inducing both humoral and cell-mediated responses to the recombinant SIV antigens.

Item Type: Article
Subjects: R Medicine
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: R.F. Xu
Date Deposited: 04 Jun 2009 22:13
Last Modified: 19 Jun 2014 15:17
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