Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure

Baillie, G.S. and Hitchcock, C.A. and Burnet, Frank R. (1996) Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure. Journal of Medical and Veterinary Mycology, 34 (5). pp. 341-347. ISSN 0268-1218. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Microsomal fractions were isolated from Aspergillus fumigatus cultures that had been treated with dimethylsulphoxide (DMSO), ethanol, benzopyrene, phenobarbital or naphthalene. All these xenobiotics increased microsomal cytochrome P-450 content. Cytochrome P-450 reductase activity remained unaffected and no alteration in the microsomal protein profile was detectable by SDS PAGE. Benzopyrene, dimethylaniline, hexobarbitol and p-nitroanisole were metabolised by A. fumigatus microsomes, while aniline, ethoxyresorufin and pentoxyresorufin were not. No xenobiotic elicited a more specific oxidation of any of the substrates. A. fumigatus microsomes also catalyzed the cell-free biosynthesis of ergosterol and quantitative analysis of assay metabolites showed that exposure to ethanol, benzopyrene or phenobarbitone in vivo resulted in enhanced ergosterol biosynthesis in vitro.

Item Type: Article
Uncontrolled keywords: Aspergillus; cytochrome P-450; ergosterol biosynthesis; xenobiotics
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: M.A. Ziai
Date Deposited: 05 Oct 2009 07:21
Last Modified: 18 Jun 2014 11:33
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