Skip to main content

Vancomycin production in batch and continuous culture

McIntyre, J.J., Bull, Alan T., Bunch, Alan William (1996) Vancomycin production in batch and continuous culture. Biotechnology and Bioengineering, 49 (4). pp. 412-420. ISSN 0006-3592. (doi:10.1002/(SICI)1097-0290(19960220)49:4<412::AID-BIT8>3.0.CO;2-S) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18679)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL


Production of the glycopeptide antibiotic vancomycin by two Amycolatopsis orientalis strains was examined in batch shake flask culture in a semidefined medium with peptone as the nitrogen source. Different growth and production profiles were observed with the two strains; specific producion (Yp/x) was threefold higher with strain ATCC 19795 than with strain NCIMB 12945. A defined medium with amino acids as the nitrogen source was developed by use of the Plackett-Burman statistical screening method. This technique identified certain amino acids (glycine, phenylalanine, tyrosine, and arginine) that gave significant increased specific production, whereas phosphate was identified as inhibitory for high specific vancomycin production. Experiments made with the improved medium and strain ATCC 19795 showed that vancomycin production kinetics were either growth dissociated or growth associated, depending on the amino acid concentration. In chemostat culture at a constant dilution rate (0.087 h(-1)), specific vancomycin production rate (q(vancomycin)) decreased linearly as the medium phosphate concentration was increased from 2 to 8 mM. In both phosphate and glucose limited chemostats, q(vancomycin) was a function of specific growth rate; the maximum value was observed at D = 0.087 h(-1) (52% of the maximum specific growth rate). Under phosphate limited growth conditions, qvani, was threefold higher (0.37 mg/g dry weight/h) than under glucose limitation (0.12 mg/g dry weight/h).

Item Type: Article
DOI/Identification number: 10.1002/(SICI)1097-0290(19960220)49:4<412::AID-BIT8>3.0.CO;2-S
Uncontrolled keywords: Amycolatopsis orientalis; vancomycin production; chemostat culture; phosphate inhibition
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: F.D. Zabet
Date Deposited: 24 Jun 2009 15:27 UTC
Last Modified: 16 Nov 2021 09:56 UTC
Resource URI: (The current URI for this page, for reference purposes)
Bull, Alan T.:
  • Depositors only (login required):