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The purification and characterization of a novel D(-)-specific carbamoylase enzyme from an Agrobacterium sp.

Louwrier, Ariel, Knowles, Christopher J. (1996) The purification and characterization of a novel D(-)-specific carbamoylase enzyme from an Agrobacterium sp. Enzyme and Microbial Technology, 19 (8). pp. 562-571. ISSN 0141-0229. (doi:10.1016/0141-0229(95)00044-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18649)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1016/0141-0229(95)00044-5

Abstract

A carbamoylase enzyme was purified from a cell-free extract of Agrobacterium sp. with an overall yield of 81%. It was judged to be homogenous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a subunit molecular weight of 38,000 daltons. Further studies on the native enzyme suggested that the active enzyme was present as a dimer, with a pl of 5.5. It was able to cleave a variety of N-carbamoyl substrates, but was strictly D(-) specific. It was found to have a K-m of 0.82 mM and a V-max of 31 U mg(-1) for D(-) N-carbamoyl hydroxyphenylglycine in the presence of 10 mM dithiothreitol. It showed no metal ion requirements but was inhibited by iodoacetic acid and iodoacetamide, both thiol reagents. The N-terminal amino acid sequence of the enzyme was elucidated.

Item Type: Article
DOI/Identification number: 10.1016/0141-0229(95)00044-5
Uncontrolled keywords: carbamoylase; hydantoinase; N-carbamoyl hydroxyphenylglycine; hydroxyphenylglycine; hydroxyphenyl hydantoin
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: F.D. Zabet
Date Deposited: 29 Jun 2009 10:08 UTC
Last Modified: 16 Nov 2021 09:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18649 (The current URI for this page, for reference purposes)

University of Kent Author Information

Knowles, Christopher J..

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