Skip to main content

Uptake of liposomes which incorporate a glycopeptide fraction of asialofetuin by HepG(2) cells

Kallinteri, Paraskevi, Papadimitriou, Evangelia, Antimisiaris, Sophia G. (2001) Uptake of liposomes which incorporate a glycopeptide fraction of asialofetuin by HepG(2) cells. Journal of Liposome Research, 11 (2-3). pp. 175-193. ISSN 0898-2104. (doi:10.1081/LPR-100108461) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18612)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1081/LPR-100108461

Abstract

We examined the interaction between liposomes which incorporate a fraction triantennary glycopeptide (AF) of asialofetuin and human hepatoma cells (HepG(2)) in vitro. HepG(2) cells are known to express the asialoglycoprotein receptor.

Results from both, morphological and quantitative studies, demonstrate that AF(2)/C-16 and especially AF(2)/C-18-liposomes are bound and taken up by the cells by a galactose specific mechanism. The AF(2)/C-12-liposomes-which were previously found to incorporate low amounts of ligand in a non-stable way-were taken up by the cells in amounts similar to those of the control liposomes (without ligand) while this uptake was not reduced by galactose and therefore possibly non-specific. The intracellular localization of AF(2)/C-18-liposomes was further evidenced by intracellular acidification using NH4Cl. These conclusions. justify the importance of further in vivo studies in order to demonstrate the capability of the proposed system to target hepatocytes.

Item Type: Article
DOI/Identification number: 10.1081/LPR-100108461
Additional information: 496RK Times Cited:2 Cited References Count:39
Uncontrolled keywords: fetuin liposome hepg(2) cells asialoglycoprotein receptor targeting hepatic asialoglycoprotein receptor labeled liposomes rabbit hepatocytes high-affinity binding endocytosis assay line galactosides glycosides
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Paraskevi Kallinteri
Date Deposited: 27 May 2009 15:25 UTC
Last Modified: 28 May 2019 13:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18612 (The current URI for this page, for reference purposes)
  • Depositors only (login required):