Stability of SUV liposomes in the presence of cholate salts and pancreatic lipases: effect of lipid composition

Kokkona, Maria and Kallinteri, Paraskevi and Fatouros, Dimitrios and Antimisiaris, Sophia G. (2000) Stability of SUV liposomes in the presence of cholate salts and pancreatic lipases: effect of lipid composition. European Journal of Pharmaceutical Sciences, 9 (3). pp. 245-252. ISSN 0928-0987. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The effect of bile salts (sodium cholate and sodium taurocholate), and pancreatic lipases on the structural integrity of SW liposomes of different lipid compositions was studied. Liposomal membrane integrity was judged by bile salt or pancreatin-induced release of vesicle encapsulated 5,6-carboxyfluorescein, and vesicle size distribution before and after incubations. Bile salt concentration was 10 mM, while a saturated solution of pancreatin (mixed with equal volume of liposomes) was utilized. Results agree with earlier studies, demonstrating the instability of liposomes composed of lipids with low transition temperatures (PC and DMPC) in presence of cholates. Addition of cholesterol (1:1 lipid:chol molar ratio) does not substantially increase the encapsulated molecule retention. Nevertheless, liposomes composed of lipids with high transition temperatures (DPPC, DSPC and SM), retain significantly higher amounts of encapsulated material, under all conditions studied. Furthermore, the vesicles formed by mixing cholesterol with these lipids will possibly be sufficiently stable in the gastrointestinal tract for long periods of time. Sizing results reveal that in most cases release of encapsulated molecules is mainly caused by their leakage through holes formed on the Lipid bilayer. However, in stearylamine containing DPPC and DSPC vesicles, the cholate-induced drastic decrease in vesicle size suggests total liposome disruption as the possible mechanism of encapsulated material immediate release. (C) 2000 Elsevier Science B.V. All rights reserved.

Item Type: Article
Additional information: 293TZ Times Cited:28 Cited References Count:37
Uncontrolled keywords: liposome stability bile salt pancreatic lipases oral administration in-vitro stability phosphatidylcholine liposomes phase-transitions phospholipid-vesicles coated liposomes rats cholesterol calcitonin absorption membranes
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Science Technology and Medical Studies > Medway School of Pharmacy
Depositing User: Paraskevi Kallinteri
Date Deposited: 24 Jun 2009 10:55
Last Modified: 08 Apr 2014 08:35
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