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Activation of translation initiation factor eIF2B by insulin requires phosphatidyl inositol 3-kinase

Welsh, Gavin I., Stokes, Christa M., Wang, Xuemin, Sakaue, Hiroshi, Ogawa, Wataru, Kasuga, Masato, Proud, Christopher G. (1997) Activation of translation initiation factor eIF2B by insulin requires phosphatidyl inositol 3-kinase. FEBS Letters, 410 (2-3). pp. 418-422. ISSN 0014-5793. (doi:10.1016/S0014-5793(97)00579-6) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:18038)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/S0014-5793(97)00579-6

Abstract

Eukaryotic initiation factor eIF2B mediates a key regulatory step in peptide-chain initiation and is acutely activated by insulin, although it is not clear how, Inhibitors of phosphatidylinositide 3-kinase blocked activation of eIF2B, although rapamycin, which inhibits the p70 S6 kinase pathway, did not, Furthermore, a dominant negative mutant of PI 3-kinase also prevented activation of eIF2B, while a Sos-mutant, which blocks MAP kinase activation, did not. The data demonstrate that a pathway distinct from MAP and p70 S6 kinases regulates eIF2B, Glycogen synthase kinase-3 (GSK-3) phosphorylates and inactivates eIF2B, In all cases, eIF2B and GSK-3 were regulated reciprocally, Dominant negative PI 3-kinase abolished the insulin-induced inhibition of GSK-3, These data strongly support the hypothesis that insulin activates eIF2B through a signalling pathway involving PI 3-kinase and inhibition of GSK-3. (C) 1997 Federation of European Biochemical Societies.

Item Type: Article
DOI/Identification number: 10.1016/S0014-5793(97)00579-6
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: T.J. Sango
Date Deposited: 29 Apr 2009 12:55 UTC
Last Modified: 28 May 2019 13:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/18038 (The current URI for this page, for reference purposes)
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