Lagutin, Oleg V., Dobrovolsky, Vasily N., Vinogradova, T.V., Kyndiakov, B.N., Khodarovich, Y.M., Jenkins, Nigel, James, David C., Markham, Nicola H., Larionov, Oleg A. (1999) Efficient human IFN-gamma expression in the mammary gland of transgenic mice. Journal of Interferon and Cytokine Research, 19 (2). pp. 137-144. ISSN 1079-9907. (doi:10.1089/107999099314289) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:16894)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: https://doi.org/10.1089/107999099314289 |
Abstract
Two hybrid genes (BLG-HuIFN-gamma 2 and BLG-HuIFN-gamma 3) were constructed on the basis of sheep beta-lactoglobulin (BLG) and human interferon-gamma (HuIFN-gamma) gene sequences. They were used to direct HuIFN-gamma synthesis in the mammary gland of transgenic mice. HuIFN-gamma was efficiently produced in the mammary gland of transgenic mice. BLG-HuIFN-gamma 2 transgenic females expressed HuIFN-gamma in the milli at concentrations up to 570 mg/ml, and BLG-HuIFN-gamma 3 transgenic females expressed up to 350 mg/ml. All females carrying the BLG-HuIFN-gamma 3 gene expressed HuIFN-gamma in their milk. No significant changes were observed in the HuIFN-gamma expression level during the lactation period, Using RT-PCR analysis, ectopic expression for both hybrid genes was found in transgenic mice. Despite ectopic expression of HuIFN-gamma in transgenic mice, their development and pregnancy were normal. The heritability of the HuIFN-gamma expression lever in milk was demonstrated up to the F-2 generation. This work demonstrates that hybrid genes have the potential to develop in transgenic domestic animals producing HuIFN-gamma in milk.
Item Type: | Article |
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DOI/Identification number: | 10.1089/107999099314289 |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | I.T. Ekpo |
Date Deposited: | 14 Apr 2009 15:14 UTC |
Last Modified: | 05 Nov 2024 09:52 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/16894 (The current URI for this page, for reference purposes) |
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