A chromogenic substrate assay kit for factor XII: Evaluation and use for the measurement of factor XII levels in cardiopulmonary bypass patients

Gallimore, Michael J. and Jones, David Wynne and Wendel, Hans P. (1999) A chromogenic substrate assay kit for factor XII: Evaluation and use for the measurement of factor XII levels in cardiopulmonary bypass patients. Thrombosis Research, 94 (2). pp. 103-109. ISSN 0049-3848. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/S0049-3848(98)00202-3

Abstract

Factor XII levels were determined in plasma samples from 75 patients before undergoing aortocoronary bypass grafting and from 40 healthy age-matched donors by using a microtitre plate adaptation of a new chromogenic peptide substrate assay kit for factor XII. The chromogenic peptide substrate assay values for factor XII correlated well with those obtained in clotting (r=0.90; y= 15.811+0.8236x) and immunochemical (r=0.88; y=17.90+0.817x) assays in the normal donor samples. Factor XII levels in the patients were significantly lower than those in the normal donors (83.3+/-23.2% versus 103.4+/-23.1; p=0.004), and nine patients (12%) had factor XII values below 50% compared with only one of the normal donors (2.5%). Factor XII levels and kallikrein-like activities (a measure of contact system activation) were followed before, during, and one day after cardiopulmonary bypass in 20 patients. Factor XII levels were significantly reduced, and kallikrein-like activities significantly elevated after 5 and 30 minutes cardiopulmonary bypass. One day after monary bypass both factor XII levels and kallikrein-like activities were significantly lower than preoperation values. (C) 1999 Elsevier Science Ltd. All rights reserved.

Item Type: Article
Uncontrolled keywords: factor XII; chromogenic substrate assay; cardiopulmonary bypass
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: I.T. Ekpo
Date Deposited: 02 May 2009 17:56
Last Modified: 01 May 2014 13:59
Resource URI: https://kar.kent.ac.uk/id/eprint/16792 (The current URI for this page, for reference purposes)
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