Fate of phenanthrene, pyrene and benzo[a]pyrene during biodegradation of crude oil added to two soils

Smith, Michael J. and Lethbridge, Gordon and Burns, Richard G. (1999) Fate of phenanthrene, pyrene and benzo[a]pyrene during biodegradation of crude oil added to two soils. FEMS Microbiology Letters, 173 (2). pp. 445-452. ISSN 03781097. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The release of (CO2)-C-14 from 9-[C-14]phenanthrene, 4,5,9,10-[C-14]pyrene and 7-[C-14]benzo[a]pyrene, added to Brent/Fortes crude oil and mixed into a pristine sand soil (0.40% organic C) and a pristine organic soil (22.9% organic C), was determined. After 244 days at 25 degrees C, 11.1 +/- 3.5% (sand) and 17.1 +/- 0.30% (organic) phenanthrene-C-14 and 9.77 +/- 2.8% (sand) and 5.56 +/- 1.4% (organic) benzo[a]pyrene-C-14 was released. After 210 days, 3.65 +/- 0.5% (sand) and 4.43 +/- 0.33% (organic) pyrene-C-14 was released. Inoculation of these two soils with DC1 and PD2 (bacteria capable of accelerating the phenanthrene and pyrene mineralisation in soil in the absence of crude oil) either at day 0 or after release as (CO2)-C-14 by indigenous degraders had ceased, failed to increase or initiate further mineralisation. Thus, aged PAH residues were non-bioavailable to these metabolically competent degrading microorganisms. At the end of the first period of incubation (210 days or 244 days), the total aromatic hydrocarbons recovered using Soxhlet extraction was 0.18% (sand) and 42.8% (organic) compared with approximately 100% from bio-inhibited soils. This confirmed that the indigenous microbiological activity not only caused a limited amount of PAH mineralisation but also reduced the extractability of residues, possibly due to the generation of metabolites which were chemisorbed and bound (and non extractable) in 'aged' soils.

Item Type: Article
Uncontrolled keywords: polycyclic aromatic hydrocarbons; bioavailability; ageing; chemisorption
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: F.D. Zabet
Date Deposited: 24 Mar 2009 12:55
Last Modified: 24 Apr 2014 08:12
Resource URI: https://kar.kent.ac.uk/id/eprint/16639 (The current URI for this page, for reference purposes)
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