Actomyosin regulatory properties of yeast tropomyosin are dependent upon N-terminal modification

Maytum, Robin and Geeves, Michael A. and Konrad, Manfred (2000) Actomyosin regulatory properties of yeast tropomyosin are dependent upon N-terminal modification. Biochemistry, 39 (39). pp. 11913-11920. ISSN 0006-2960. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The yeast tropomyosin 1 gene (TPM1) encodes the major isoform of the two tropomyosins (Tm) found in yeast. The gene has been expressed in E. coli and the protein purified. The gene product (yTm1) is a 199-amino acid protein that has a low affinity for actin compared to the native yTm1 purified from yeast. Mass spectrometry shows that the native protein is acetylated while the recombinant protein is not. A series of yTm1 N-terminal constructs were made with either an Ala-Ser dipeptide extension previously shown to restore actin binding to skeletal muscle Tm or the natural extension found in fibroblast Tm 5a/b. All constructs bound actin tightly and showed similar CD spectra and thermal stability. All constructs induced cooperativity in the equilibrium binding of myosin subfragment 1, to actin but the binding curves differed significantly between the constructs. The apparent cooperative unit size (n) and closed/open equilibrium (K-T) were determined using a fluorescence titration technique [Maytum et al. (1998) Biophys, J. 74, A347]. The data could be accounted for by changes in K-T (0.1-1) With no change in n, Values of n were approximately twice the structural unit size (5 actin sites). The presence of yTm on actin had little effect upon the overall affinity of S1 for actin despite showing an ability to regulate the acto-myosin interaction, These results show that the short yTm can aid our understanding of actomyosin regulation and that the N-terminus of Tm has a major influence upon its regulatory properties.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: O.O. Odanye
Date Deposited: 17 Mar 2009 15:58
Last Modified: 21 May 2014 07:37
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