Rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes

Colquhoun, Joy A. and Zulu, Joseph and Goodfellow, Michael and Horikoshi, Koki and Ward, Alan C. and Bull, Alan T. (2000) Rapid characterisation of deep-sea actinomycetes for biotechnology screening programmes. Antonie Van Leeuwenhoek, 77 (4). pp. 359-367. ISSN 0003-6072. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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A continual need in natural product discovery is dereplication, that is the ability to exclude previously tested microorganisms from screening programmes. Whole-cell fingerprinting techniques offer an ideal solution to this problem because of their rapidity and reproducibility, dependence on small samples, and automation. One such technique, Curie-point pyrolysis mass spectrometry (PyMS), has been deployed for the characterisation of a unique collection of actinomycetes recovered from Pacific Ocean sediments approximately 2000 to 6500 m below sea level. This paper addresses the question: to what extent are pyrogroups, defined on the basis of PyMS fingerprinting, related to classifications derived from more conventional microbial systematics? A collection of 44 randomly chosen deep-sea rhodococci were coded and subjected to a double-blind PyMS and numerical taxonomic (NT) analysis; the latter sorted the strains into clusters (taxospecies) using large sets of equally weighted phenotypic data. At the end of the experiment the codes were disclosed and the NT classification shown to generate 6 homogeneous clusters corresponding to different deep-sea sites. The matching of these clusters with the resulting pyrogroups was very high with an overall congruence of nearly 98%. Thus, PyMS characterisation is directly ascribable to the phenotypic variation being sought for biotechnology screens. Moreover, the exquisite discriminatory power of PyMS readily revealed infraspecific diversity in these industrially important bacteria.

Item Type: Article
Uncontrolled keywords: actinomycetes; deep-sea; dereplication; pyrolysis mass spectrometry; screening
Subjects: Q Science > Q Science (General)
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: P. Ogbuji
Date Deposited: 01 Apr 2009 17:04
Last Modified: 22 Apr 2014 11:41
Resource URI: https://kar.kent.ac.uk/id/eprint/16301 (The current URI for this page, for reference purposes)
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