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Search and discovery strategies for biotechnology: The paradigm shift

Bull, Alan T. and Ward, Alan C. and Goodfellow, Michael (2000) Search and discovery strategies for biotechnology: The paradigm shift. Microbiology and Molecular Biology Reviews, 64 (3). 573-+. ISSN 1092-2172. (doi:10.1128/MMBR.64.3.573-606.2000) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:16180)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
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Profound changes are occurring in the strategies that biotechnology-based industries are deploying in the search for exploitable biology and to discover new products and develop new or improved processes. The advances that have been made in the past decade in areas such as combinatorial chemistry, combinatorial biosynthesis, metabolic pathway engineering, gene shuffling, and directed evolution of proteins have caused some companies to consider withdrawing from natural product screening. In this review we examine the paradigm shift from traditional biology to bioinformatics that is revolutionizing exploitable biology. We conclude that the reinvigorated means of detecting novel organisms, novel chemical structures, and novel biocatalytic activities will ensure that natural products will continue to be a primary resource for biotechnology. The paradigm shift has been driven by a convergence of complementary technologies, exemplified by DNA sequencing and amplification genome sequencing and annotation, proteome analysis and phenotypic inventorying, resulting in the establishment of huge databases that can be mined in order to generate useful knowledge such as the identity and characterization of organisms and the identity of biotechnology targets. Concurrently there have been major advances in understanding the extent of microbial diversity, how uncultured organisms might be grown, and how expression of the metabolic potential of microorganisms can be maximized. The integration of information from complementary databases presents a significant challenge. Such integration should facilitate answers to complex questions involving sequence, biochemical, physiological, taxonomic, and ecological information of the sort posed in exploitable biology. The paradigm shift which we discuss is not absolute in the sense that it will replace established microbiology; rather, it reinforces our view that innovative microbiology is essential for releasing the potential of microbial diversity for biotechnology penetration throughout industry Various of these issues are considered with reference to deep-sea microbiology and biotechnology.

Item Type: Review
DOI/Identification number: 10.1128/MMBR.64.3.573-606.2000
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: P. Ogbuji
Date Deposited: 13 Apr 2009 18:41 UTC
Last Modified: 16 Nov 2021 09:54 UTC
Resource URI: (The current URI for this page, for reference purposes)

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