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Degradation and mineralization of 2-chloro-, 3-chloro- and 4-chlorobiphenyl by a newly characterized natural bacterial strain isolated from an electrical transformer fluid-contaminated soil

Ilori, Matthew O., Robinson, Gary K., Adebusoye, Sunday A. (2008) Degradation and mineralization of 2-chloro-, 3-chloro- and 4-chlorobiphenyl by a newly characterized natural bacterial strain isolated from an electrical transformer fluid-contaminated soil. Journal of Environmental Sciences-China, 20 (10). pp. 1250-1257. ISSN 1001-0742. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:15705)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)

Abstract

A bacterium classified as Achromobacter xylosoxidans strain IR08 by phenotypic typing coupled with 16S rRNA gene analysis was isolated from a soil contaminated with electrical transformer fluid for over sixty years using Aroclor 1221 as an enrichment substrate. The substrate utilization profiles revealed that IR08 could grow on all three monochlorobiphenyls (CBs), 2,4'- and 4,4'-dichlorobiphenyl as well as 2-chlorobenzoate (2-CBA), 3-CBA, 4-CBA, and 2,3-dichlorobenzoate. Unusually, growth was poorly sustained on biphenyl and benzoate. In growth experiments, IR08 degraded all CBS (0.27 mmol/L) in less than 96 h with concomitant stoichiometric release of inorganic chloride and growth yields were 2-3 times higher than those observed on biphenyl. In contrast to most of the chlorobiphenyl-degrading strains described in the literature, which are reported to form CBA, no metabolite was identified in the culture broth by HPLC analysis. When co-incubated with respective CBs and biphenyl, strain IR08 preferentially utilized the chlorinated analogues in less than 96 h while it took another 264 h before 90% of the initially supplied biphenyl could be degraded. The promotion of co-metabolic transformation of halogenated substrates by the inclusion of their non-halogenated derivatives may not therefore, result in universal benefits.

Item Type: Article
Uncontrolled keywords: aerobic degradation; chlorobenzoates; mineralization; monochlorobiphenyls; natural strain; polychlorinated biphenyls
Depositing User: Jane Griffiths
Date Deposited: 22 Apr 2009 10:56 UTC
Last Modified: 06 Feb 2020 04:03 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/15705 (The current URI for this page, for reference purposes)
Robinson, Gary K.: https://orcid.org/0000-0003-2660-7778
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