Repetitive flanking sequences (ReFS): novel molecular markers from microsatellite families

Anderson, Sarah J. and Gould, Phil and Freeland, Joanna R. (2007) Repetitive flanking sequences (ReFS): novel molecular markers from microsatellite families. Molecular Ecology Notes, 7 (3). pp. 374-376. ISSN 1471-8278. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Although microsatellite markers have become exceedingly popular in molecular studies of wild organisms, their development in some taxonomic groups is challenging. This is partly because of repetitive flanking sequences, which lead to the simultaneous amplification of alleles from multiple loci. Until now, these microsatellite DNA families have been considered unsuitable for population genetics studies, but here we describe our development of these repetitive flanking sequences (ReFS) as novel molecular markers. We illustrate the utility of these markers by using them to address an outstanding taxonomic question in the moth genus Schrankia.

Item Type: Article
Uncontrolled keywords: microsatellites; molecular markers; ReFS; repetitive DNA; Schrankia LEPIDOPTERA; DNA
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Faculties > Social Sciences > School of Anthropology and Conservation > Biodiversity Conservation Group
Depositing User: Stephen Holland
Date Deposited: 19 Dec 2007 18:56
Last Modified: 11 Apr 2014 11:29
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