Clark, Richard J., Nyitrai, Miklos, Webber, Martin, Geeves, Michael A. (2003) Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin. Journal of Muscle Research and Cell Motility, 24 (4-6). pp. 315-321. ISSN 0142-4319. (doi:10.1023/A:1025481908301) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:13192)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1023/A:1025481908301 |
Abstract
The detailed kinetic analysis of novel myosin motors is often limited by the quantity of stable protein available for study. We show here that the use of coumarin based fluorescent ADP analogues allows the assay of ADP affinities and dissociation rate constants in a flash photolysis apparatus using microg quantities of the rabbit muscle myosin S1. We go on to use the analogues to characterise two other rat muscle myosin S1 and the motor domain of Dictyostelium cytoplasmic myosin II. The results show that the fluorescence change for the binding of a coumarin based ADP analogue to a myosin motor domain is variable in sign as well as amplitude for the different proteins. The analysis also provided estimates of the affinities of caged-ATP for S1 which were < or = 10 microM for muscle S1s and > 200 microM for the non-muscle myosin.
Item Type: | Article |
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DOI/Identification number: | 10.1023/A:1025481908301 |
Additional information: | 0142-4319 (Print) Journal Article |
Uncontrolled keywords: | Adenosine Diphosphate/analogs & derivatives/metabolism/pharmacology Animals Coumarins/metabolism/pharmacology Myosins/administration & dosage/*metabolism Nucleotides/*metabolism Protein Binding/drug effects/physiology Rabbits Rats Research Support, Non-U.S. Gov't |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Michael Geeves |
Date Deposited: | 14 Mar 2009 16:09 UTC |
Last Modified: | 16 Nov 2021 09:51 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/13192 (The current URI for this page, for reference purposes) |
Geeves, Michael A.: |
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