Nuclear organisation in totipotent human nuclei and its relationship to chromosomal abnormality

Finch, Katie A. and Fonseka, Gothami and Ioannou, Dimitris and Hickson, Nicholas and Barclay, Zoe and Chatzimeletiou, Katerina and Mantzouratou, Anna and Handyside, Alan H and Delhanty, Joy and Griffin, Darren K. (2008) Nuclear organisation in totipotent human nuclei and its relationship to chromosomal abnormality. Journal of Cell Science, 121 (5). pp. 655-663. ISSN 0021-9533 . (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Studies of nuclear organisation, most commonly determining the nuclear location of chromosome territories and individual loci, have furthered our understanding of nuclear function, differentiation and disease. In this study, by examining eight loci on different chromosomes, we tested hypotheses that: (1) totipotent human blastomeres adopt a nuclear organisation akin to that of committed cells; (2) nuclear organisation is different in chromosomally abnormal blastomeres; and (3) human blastomeres adopt a ;chromocentre' pattern. Analysis of in vitro fertilisation (IVF) conceptuses permits valuable insight into the cell biology of totipotent human nuclei. Here, extrapolations from images of preimplantation genetic screening (PGS) cases were used to make comparisons between totipotent blastomeres and several committed cells, showing some differences and similarities. Comparisons between chromosomally abnormal nuclei and those with no detected abnormality (NDA) suggest that the former display a significant non-random pattern for all autosomal loci, but there is a less distinct, possibly random, pattern in 'NDA' nuclei. No evidence was found that the presence of an extra chromosome is accompanied by an altered nuclear location for that chromosome. Centromeric loci on chromosomes 15 and 16 normally seen at the nuclear periphery were mostly centrally located in aneuploid cells, providing some evidence of a 'chromocentre'; however, the chromosome-18 centromere was more peripheral, similar to committed cells. Our results provide clues to the nature of totipotency in human cells and might have future applications for preimplantation diagnosis and nuclear transfer.

Item Type: Article
Additional information: jcs.025205 0021-9533
Uncontrolled keywords: nuclear organisation; chromosome; preimplantation human embryos
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Darren Griffin
Date Deposited: 27 Mar 2009 18:15
Last Modified: 02 May 2014 13:10
Resource URI: (The current URI for this page, for reference purposes)
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