Heterologous Expression of Ion Channels in Mammalian Cell Lines

Mathie, Alistair and Veale, Emma L. and Holden, Robyn G. (2021) Heterologous Expression of Ion Channels in Mammalian Cell Lines. In: Patch Clamp Electrophysiology. Springer Nature, New York, USA, pp. 51-65. ISBN 978-1-0716-0820-3. E-ISBN 978-1-0716-0818-0. (doi:10.1007/978-1-0716-0818-0_3) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:114875)

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Official URL: https://doi.org/10.1007/978-1-0716-0818-0_3

Abstract

Heterologous expression of recombinant ion channel subunits in mammalian cell lines allows for characterization of their functional properties and pharmacological regulation. In this chapter, we describe methods for thawing, refreezing, passaging, cell culture, and transfection of tsA201 cells suitable for electrophysiology and imaging experiments. Furthermore, we discuss the strengths and limitations of using these methods.

Item Type:	Book section
DOI/Identification number:	10.1007/978-1-0716-0818-0_3
Institutional Unit:	Schools > Medway School of Pharmacy
Former Institutional Unit:	There are no former institutional units.
Funders:	University of Kent (https://ror.org/00xkeyj56)
Depositing User:	Emma Veale
Date Deposited:	12 May 2026 21:37 UTC
Last Modified:	13 May 2026 14:05 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/114875 (The current URI for this page, for reference purposes)

University of Kent Author Information

Mathie, Alistair.

Creator's ORCID:	https://orcid.org/0000-0001-6094-2890
CReDIT Contributor Roles:

Veale, Emma L..

Creator's ORCID:	https://orcid.org/0000-0002-6778-9929
CReDIT Contributor Roles:

Holden, Robyn G..

Creator's ORCID:
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