The coding and long noncoding single-cell atlas of the developing human fetal striatum

Bocchi, Vittoria Dickinson, Conforti, Paola, Vezzoli, Elena, Besusso, Dario, Cappadona, Claudio, Lischetti, Tiziana, Galimberti, Maura, Ranzani, Valeria, Bonnal, Raoul J. P., De Simone, Marco, and others. (2021) The coding and long noncoding single-cell atlas of the developing human fetal striatum. Science, 372 (6542). ISSN 0036-8075. (doi:10.1126/science.abf5759) (KAR id:114676)

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Official URL: https://doi.org/10.1126/science.abf5759

Abstract

Deciphering how the human striatum develops is necessary for understanding the diseases that affect this region. To decode the transcriptional modules that regulate this structure during development, we compiled a catalog of 1116 long intergenic noncoding RNAs (lincRNAs) identified de novo and then profiled 96,789 single cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (D1- and D2-MSNs) arise from a common progenitor and that lineage commitment is established during the postmitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type–specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. This work delineates the cellular hierarchies governing MSN lineage commitment.

Item Type:	Article
DOI/Identification number:	10.1126/science.abf5759
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Institutional Unit:	Schools > Medway School of Pharmacy
Former Institutional Unit:	There are no former institutional units.
Funders:	National Institute for Health Research (https://ror.org/0187kwz08) Wellcome Trust (https://ror.org/029chgv08)
Depositing User:	Romina Vuono
Date Deposited:	11 May 2026 07:30 UTC
Last Modified:	14 May 2026 08:34 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/114676 (The current URI for this page, for reference purposes)

University of Kent Author Information

Vuono, Romina.

Creator's ORCID:	https://orcid.org/0000-0002-5162-3422
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