Schubert, Heidi L., Raux-Deery, Evelyne, Warren, Martin J., Wilson, Keith S. (2001) Optimization of Met8p crystals through protein-storage buffer manipulation. Acta Crystallographica Section D-Biological Crystallography, 57 (6). pp. 867-869. ISSN 0907-4449. (doi:10.1107/S0907444901004619) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:11063)
| The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
| Official URL: http://dx.doi.org/10.1107/S0907444901004619 |
Abstract
Sirohaem, the prosthetic group of assimilatory sulfite and nitrite reductases, is a modified tetrapyrrole that belongs to the same fraternity of metallo-prosthetic groups as haem, chlorophyll, cobalamin and coenzyme F430 [Warren & Scott (1990), Trends Biochem Sci. 15, 486-491]. In Saccharomyces cerevisiae, the last step in the biosynthesis of sirohaem involves Met8p, a bifunctional enzyme responsible for both the NAD(+)-dependent dehydrogenation of the corrin ring and ferrochelation. Optimization of the protein storage buffer according to the results of crystallization trials resulted in a more monodisperse protein solution. Crystals were grown that diffracted to 2.1 A.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1107/S0907444901004619 |
| Uncontrolled keywords: | Buffers Crystallization Crystallography, X-Ray *Ferrochelatase Fungal Proteins/*chemistry Multienzyme Complexes/chemistry Protein Conformation Saccharomyces cerevisiae/chemistry/*metabolism *Saccharomyces cerevisiae Proteins |
| Subjects: | Q Science |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Martin Warren |
| Date Deposited: | 12 Oct 2009 08:12 UTC |
| Last Modified: | 05 Nov 2024 09:44 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/11063 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Raux-Deery, Evelyne.
| Creator's ORCID: |
 https://orcid.org/0000-0002-8189-678X
|
|---|---|
| CReDIT Contributor Roles: |
Warren, Martin J..
| Creator's ORCID: |
https://orcid.org/0000-0002-6028-6456
|
|---|---|
| CReDIT Contributor Roles: |
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