Skip to main content

Structural insights into the soluble form of CLIC1 and its mechanism of membrane insertion

Cassar, Joseph (2023) Structural insights into the soluble form of CLIC1 and its mechanism of membrane insertion. Doctor of Philosophy (PhD) thesis, University of Kent,. (doi:10.22024/UniKent/01.02.103696) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:103696)

PDF
Language: English

Restricted to Repository staff only until October 2026.
Contact us about this Publication
[thumbnail of 36CASSAR2023PHDFINAL.pdf]
Official URL:
https://doi.org/10.22024/UniKent/01.02.103696

Abstract

The chloride intracellular channel (CLIC) family has a unique characteristic which enables this human protein to be expressed as a soluble protein, however with an increase in the levels of intracellular zinc ions it can insert into the membrane. In the membrane, CLIC proteins have chloride ion channel activity. To date, the exact mechanism of CLIC1 insertion into the membrane is unknown.

Utilising biophysical techniques such as SAXS and NMR, we have identified that CLIC can exist in an ensemble of structures within solution that likely aid its insertion into the membrane. Further studies demonstrate that zinc can bind to CLIC1 and alters the relaxation properties of regions in the N-terminal domain that surround the predicted transmembrane region. We demonstrate that zinc is able to further promote higher order oligomeric states which would aid membrane insertion as previous literature suggests chloride ion channel conductance occurs in a tetrameric arrangement.

Mutations to residues impacted by zinc binding are able to disrupt the equilibrium of oligomeric states in solution, further pointing to the importance of these regions and residues for the metamorphic activity of CLIC1. A more detailed understanding of the mechanism of insertion of CLIC1, will aid pharmacological development of inhibitors for CLIC1. CLIC1 is upregulated in the membrane bound form in cancers such as glioblastoma. Inhibition of this mechanistic switch will provide a novel drug target for many cancers with poor prognosis.

Item Type: Thesis (Doctor of Philosophy (PhD))
Thesis advisor: Ortega-Roldan, Jose
DOI/Identification number: 10.22024/UniKent/01.02.103696
Uncontrolled keywords: NMR, Proteins, Structural Biology
Subjects: Q Science > QH Natural history > QH581.2 Cell Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: University of Kent (https://ror.org/00xkeyj56)
SWORD Depositor: System Moodle
Depositing User: System Moodle
Date Deposited: 06 Nov 2023 16:10 UTC
Last Modified: 08 Nov 2023 12:42 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/103696 (The current URI for this page, for reference purposes)

University of Kent Author Information

Cassar, Joseph.

Creator's ORCID:
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.