2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster

Kuniyoshi, Taís M., Gonzalez, Andres, Lopez-Gomollon, Sara, Valladares, Ana, Bes, M. Teresa, Fillat, Maria F., Peleato, M. Luisa (2011) 2-oxoglutarate enhances NtcA binding activity to promoter regions of the microcystin synthesis gene cluster. FEBS Letters, 585 (24). pp. 3921-3926. ISSN 0014-5793. (doi:10.1016/j.febslet.2011.10.034) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:103515)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: https://doi.org/10.1016/j.febslet.2011.10.034

Abstract

The binding affinity of NtcA towards promoter regions of the microcystin gene cluster from Microcystis aeruginosa PCC 7806 has been analyzed by band-shift assay (EMSA). The key nitrogen transcriptional regulator exhibits affinity for two fragments of the bidirectional mcyDA promoter, as well as for promoter regions of mcyE and mcyH. The presence of 2-oxoglutarate increased by 2.5 fold the affinity of NtcA for the mcyA promoter region. The 2-oxoglutarate effect peaked at 0.8 mM, a physiological concentration for this compound under nitrogen-limiting conditions. The results suggest that the 2-oxoglutarate level, as a signal of the C to N balance of the cells, regulates the microcystin gene cluster.

Item Type:	Article
DOI/Identification number:	10.1016/j.febslet.2011.10.034
Subjects:	Q Science
Institutional Unit:	Schools > School of Natural Sciences > Biosciences
Former Institutional Unit:	There are no former institutional units.
Depositing User:	Sara Lopez-Gomollon
Date Deposited:	15 May 2026 14:27 UTC
Last Modified:	03 Jun 2026 14:54 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/103515 (The current URI for this page, for reference purposes)

University of Kent Author Information

Lopez-Gomollon, Sara.

Creator's ORCID:	https://orcid.org/0000-0001-8504-0340
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