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On translational control by ribosome speed in S. cerevisiae

Kazana, Eleanna, von der Haar, Tobias (2023) On translational control by ribosome speed in S. cerevisiae. Frontiers in RNA Research, 1 . Article Number 1240635. ISSN 2813-7116. (doi:10.3389/frnar.2023.1240635) (KAR id:102960)


Introduction: In addition to the widespread and well documented control of protein synthesis by translation initiation, recent evidence suggests that translation elongation can also control protein synthesis rates. One of the proposed mechanisms leading to elongation control is the interference of slow ribosome movement around the start codon with efficient translation initiation. Here we estimate the frequency with which this mode of control occurs in baker’s yeast growing in rich medium.

Methods: We interrogate published genome-wide datasets for evidence of transcripts associated with queueing small ribosomal subunits, and confirm results from these surveys using additional experimental work.

Results: Our analyses reveal that transcripts from around 20% of yeast genes show evidence of queueing ribosomes, which may be indicative of translation elongation control. Moreover, this subset of transcripts is sensitive to distinct regulatory signals compared to initiation-controlled mRNAs, and such distinct regulation occurs, for example, during the response to osmotic stress.

Discussion: Our analyses provide a first quantitative estimate for the prevalence of translational control exerted via the elongation stage in a commonly used model organism, and suggest that transcript under elongation control form a separately addressable RNA regulon.

Item Type: Article
DOI/Identification number: 10.3389/frnar.2023.1240635
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Leverhulme Trust (
Depositing User: Tobias von der Haar
Date Deposited: 26 Sep 2023 12:01 UTC
Last Modified: 11 Jan 2024 11:10 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Kazana, Eleanna.

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von der Haar, Tobias.

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