Skip to main content

Probiotics as an alternative to antibiotics in modulating the intestinal microbiota and performance of broiler chickens

Grozina, A. A., Ilina, Larisa A., Laptev, Georgi Yu., Yildirim, Elena A., Ponomareva, Ekaterina S., Filippova, Valentina A., Tyurina, Darya G., Fisinin, Vladimir I., Kochish, Ivan I., Griffin, Darren K., and others. (2023) Probiotics as an alternative to antibiotics in modulating the intestinal microbiota and performance of broiler chickens. Journal of Applied Microbiology, 134 (9). Article Number lxad213. ISSN 1364-5072. E-ISSN 1365-2672. (doi:10.1093/jambio/lxad213) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:102866)

PDF Author's Accepted Manuscript
Language: English

Restricted to Repository staff only until 14 September 2024.
Contact us about this Publication
[thumbnail of Grozina et al 2 revised.pdf]
Official URL:



Gut bacteria play an important role in poultry nutrition and the immune defense system. Changes in the intestinal microbiome affect the physiological state, metabolism and innate im-munity of poultry. The present study aimed to characterize age-related changes in the gastrointestinal tract microflora in broiler chickens, depending on supplementation of the diet with the in-feed antibiotic Stafac® 110 and a Bacillus subtilis strain-based probiotic.

Methods and results

In this regard, a comprehensive analysis of the taxonomic structure of the microbial community in the gastrointestinal tract (GIT) of broiler chickens was carried out using a molecular genetic technique of the Terminal-Restriction Fragment Length Polymorphism analysis and taking into account age dynamics and feeding treatment. A beneficial effect on the microbiological composition and body weight of broilers was observed when using the antibiotic and probiotic in compound feeds. Different bacterial communities were revealed in the duodenum and cecum and their positive impact on broiler growth was established. The results obtained shed light on the formation of GIT microflora of broiler chickens during the growing period and its changes in response to the use of the antibiotic and the probiotic.


We suggest that the implementation of the tested in-feed antibiotic and probiotic can be beneficial in regulating the intestinal microflora microbiological processes in the GIT and improving the feeding efficiency and productivity of broiler chickens.

Item Type: Article
DOI/Identification number: 10.1093/jambio/lxad213
Uncontrolled keywords: Bacillus subtilis probiotic; broiler chickens; cecum; duodenum; in-feed antibiotic; microbiome
Subjects: Q Science > QP Physiology (Living systems)
Q Science > QR Microbiology
S Agriculture > SF Animal culture
Divisions: Divisions > Division of Natural Sciences > Centre for Interdisciplinary Studies of Reproduction
Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Food Systems, Natural Resources and Environment
Depositing User: Mike Romanov
Date Deposited: 19 Sep 2023 17:18 UTC
Last Modified: 07 Nov 2023 06:41 UTC
Resource URI: (The current URI for this page, for reference purposes)
Grozina, A. A.:
Ilina, Larisa A.:
Laptev, Georgi Yu.:
Yildirim, Elena A.:
Ponomareva, Ekaterina S.:
Filippova, Valentina A.:
Tyurina, Darya G.:
Fisinin, Vladimir I.:
Kochish, Ivan I.:
Griffin, Darren K.:
Surai, Peter F.:
Romanov, Michael N.:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.