Beal, David M, Liang, Mingzhi, Brown, Ian R., Budge, James D., Burrows, Emily R., Howland, Kevin, Lee, Phoebe, Martin, Sarah, Morrell, Andrew, Nemoto-Smith, Emi, and others. (2023) Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging. Materials Advances, 4 (14). pp. 2963-2970. E-ISSN 2633-5409. (doi:10.1039/d3ma00071k) (KAR id:101867)
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Official URL: https://doi.org/10.1039/d3ma00071k |
Abstract
Bacterial microcompartments (BMCs) are proteinaceous organelle-like structures formed within bacteria, often encapsulating enzymes and cellular processes, in particular, allowing toxic intermediates to be shielded from the general cellular environment. Outside of their biological role they are of interest, through surface modification, as potential drug carriers and polyvalent antigen display scaffolds. Here we use a post-translational modification approach, using copper free click chemistry, to attach a SpyTag to a target protein molecule for attachment to a specific SpyCatcher modified BMC shell protein. We demonstrate that a post-translationally SpyTagged material can react with a SpyCatcher modified BMC and show its presence on the surface of BMCs, enabling future investigation of these structures as polyvalent antigen display scaffolds for vaccine development. This post-translational ‘click’ methodology overcomes the necessity to genetically encode the SpyTag, avoids any potential reduction in expression yield and expands the scope of SpyTag/SpyCatcher vaccine scaffolds to form peptide epitope vaccines and small molecule delivery agents.
Item Type: | Article |
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DOI/Identification number: | 10.1039/d3ma00071k |
Additional information: | For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising. |
Uncontrolled keywords: | Chemistry (miscellaneous) |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Funders: |
Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Wellcome Trust (https://ror.org/029chgv08) University of Kent (https://ror.org/00xkeyj56) |
SWORD Depositor: | JISC Publications Router |
Depositing User: | JISC Publications Router |
Date Deposited: | 29 Jun 2023 08:38 UTC |
Last Modified: | 20 Nov 2023 15:26 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/101867 (The current URI for this page, for reference purposes) |
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