Skip to main content
Kent Academic Repository

Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging

Beal, David M, Liang, Mingzhi, Brown, Ian R., Budge, James D., Burrows, Emily R., Howland, Kevin, Lee, Phoebe, Martin, Sarah, Morrell, Andrew, Nemoto-Smith, Emi, and others. (2023) Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging. Materials Advances, 4 (14). pp. 2963-2970. E-ISSN 2633-5409. (doi:10.1039/d3ma00071k) (KAR id:101867)

Abstract

Bacterial microcompartments (BMCs) are proteinaceous organelle-like structures formed within bacteria, often encapsulating enzymes and cellular processes, in particular, allowing toxic intermediates to be shielded from the general cellular environment. Outside of their biological role they are of interest, through surface modification, as potential drug carriers and polyvalent antigen display scaffolds. Here we use a post-translational modification approach, using copper free click chemistry, to attach a SpyTag to a target protein molecule for attachment to a specific SpyCatcher modified BMC shell protein. We demonstrate that a post-translationally SpyTagged material can react with a SpyCatcher modified BMC and show its presence on the surface of BMCs, enabling future investigation of these structures as polyvalent antigen display scaffolds for vaccine development. This post-translational ‘click’ methodology overcomes the necessity to genetically encode the SpyTag, avoids any potential reduction in expression yield and expands the scope of SpyTag/SpyCatcher vaccine scaffolds to form peptide epitope vaccines and small molecule delivery agents.

Item Type: Article
DOI/Identification number: 10.1039/d3ma00071k
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Uncontrolled keywords: Chemistry (miscellaneous)
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Wellcome Trust (https://ror.org/029chgv08)
University of Kent (https://ror.org/00xkeyj56)
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 29 Jun 2023 08:38 UTC
Last Modified: 20 Nov 2023 15:26 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/101867 (The current URI for this page, for reference purposes)

University of Kent Author Information

Beal, David M.

Creator's ORCID: https://orcid.org/0000-0002-1819-315X
CReDIT Contributor Roles:

Liang, Mingzhi.

Creator's ORCID:
CReDIT Contributor Roles:

Brown, Ian R..

Creator's ORCID: https://orcid.org/0000-0002-6989-4474
CReDIT Contributor Roles:

Budge, James D..

Creator's ORCID:
CReDIT Contributor Roles:

Howland, Kevin.

Creator's ORCID: https://orcid.org/0000-0003-4370-0974
CReDIT Contributor Roles:

Lee, Phoebe.

Creator's ORCID:
CReDIT Contributor Roles:

Stanley, Maria.

Creator's ORCID:
CReDIT Contributor Roles:

Smales, Christopher Mark.

Creator's ORCID: https://orcid.org/0000-0002-2762-4724
CReDIT Contributor Roles:

Warren, Martin J..

Creator's ORCID: https://orcid.org/0000-0002-6028-6456
CReDIT Contributor Roles:
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.