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Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis

de Souza, Marcelo Santos, Barcellos, Suziane Alves, Tura, Victoria, Bobrowski, Vera Lúcia, Garnero, Analía Del Valle, Gunski, Ricardo José, Griffin, Darren K., Kretschmer, Rafael (2023) Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis. Birds, 4 (2). pp. 236-244. ISSN 2673-6004. (doi:10.3390/birds4020020) (KAR id:101826)

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Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (Gallus gallus) or Zebra Finch (Taeniopygia guttata) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (Myiodynastes maculatus), Shiny Cowbird (Molothrus bonariensis), Southern House Wren (Troglodytes aedon), and Double-collared Seedeater (Sporophila caerulescens)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.

Item Type: Article
DOI/Identification number: 10.3390/birds4020020
Uncontrolled keywords: Aves; diploid number; karyotype organization; molecular cytogenetic
Subjects: Q Science
Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Coordenação de Aperfeicoamento de Pessoal de Nível Superior (
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 14 Jul 2023 15:42 UTC
Last Modified: 18 Jul 2023 08:58 UTC
Resource URI: (The current URI for this page, for reference purposes)
de Souza, Marcelo Santos:
Barcellos, Suziane Alves:
Tura, Victoria:
Gunski, Ricardo José:
Griffin, Darren K.:
Kretschmer, Rafael:
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