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Translation of in vitro -transcribed RNA therapeutics

von der Haar, Tobias, Mulroney, Thomas E., Hedayioglu, Fabio, Kurusamy, Sathishkuma, Rust, Maria, Lilley, Kathryn S., Thaventhiran, James E., Willis, Anne E., Smales, C. Mark (2023) Translation of in vitro -transcribed RNA therapeutics. Frontiers in Molecular Biosciences, 10 . Article Number 1128067. ISSN 2296-889X. (doi:10.3389/fmolb.2023.1128067) (KAR id:100172)


In vitro transcribed, modified messenger RNAs (IVTmRNAs) have been used to vaccinate billions of individuals against the SARS-CoV-2 virus, and are currently being developed for many additional therapeutic applications. IVTmRNAs must be translated into proteins with therapeutic activity by the same cellular machinery that also translates native endogenous transcripts. However, different genesis pathways and routes of entry into target cells as well as the presence of modified nucleotides mean that the way in which IVTmRNAs engage with the translational machinery, and the efficiency with which they are being translated, differs from native mRNAs. This review summarises our current knowledge of commonalities and differences in translation between IVTmRNAs and cellular mRNAs, which is key for the development of future design strategies that can generate IVTmRNAs with improved activity in therapeutic applications.

Item Type: Article
DOI/Identification number: 10.3389/fmolb.2023.1128067
Uncontrolled keywords: Molecular Biosciences, RNA therapeutic, translation, protein synthesis, translational control, RNA vaccines
Subjects: Q Science
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 18 Apr 2023 13:27 UTC
Last Modified: 03 May 2024 03:16 UTC
Resource URI: (The current URI for this page, for reference purposes)

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