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Cardiac troponin T circulates in the free, intact form in patients with kidney failure.

Fahie-Wilson, Michael N., Carmichael, David J., Delaney, Michael P., Stevens, Paul E., Hall, Elizabeth M, Lamb, Edmund J. (2006) Cardiac troponin T circulates in the free, intact form in patients with kidney failure. Clinical Chemistry, 52 (3). pp. 414-420. ISSN 0009-9147. E-ISSN 1530-8561. (doi:10.1373/clinchem.2005.062307) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:100)

Language: English

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BACKGROUND: The clinical significance of the increased concentrations of cardiac troponins observed in patients with end stage renal disease (ESRD) in the absence of an acute coronary syndrome (ACS) is controversial. One proposed explanation is that immunoreactive fragments of cardiac troponin T (cTnT) accumulate in ESRD. We used gel-filtration chromatography (GFC) to ascertain whether fragments of cTnT, which could cross-react in the commercial diagnostic immunoassay (Roche Diagnostics), were the cause of the increased cTnT in the serum of patients with ESRD. METHODS: We subjected sera from ESRD patients (n = 21) receiving dialysis and having increased cTnT concentrations to size-separation GFC. We detected cTnT in the chromatography fractions by use of the same antibodies used in the commercial assay for serum cTnT. RESULTS: In all patients, cTnT immunoreactivity eluted as a major, homogeneous peak in an identical position between the peaks of serum prolactin [relative molecular mass (Mr) 23,000] and albumin (Mr 67,000): the elution pattern of cTnT in samples obtained from ACS patients was identical to that of the ESRD patients. There was no evidence that low-molecular-mass (Mr < 23,000) cTnT fragments were the cause of the increased cTnT in the patients studied. CONCLUSIONS: The form of cTnT observed in the serum of patients with kidney failure and immunoreactive in the diagnostic assay is predominantly the free intact form, as in patients with ACS. Our data are consistent with the view that circulating cTnT in renal failure reflects cardiac pathology.

Item Type: Article
DOI/Identification number: 10.1373/clinchem.2005.062307
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 19 Dec 2007 17:57 UTC
Last Modified: 16 Nov 2021 09:39 UTC
Resource URI: (The current URI for this page, for reference purposes)

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