A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain.

Mason, Maria G., Holladay, Rebecca S., Nicholls, Peter J., Shepherd, Mark, Cooper, Chris E (2008) A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain. Methods in Enzymology, 437 . pp. 135-159. ISSN 0076-6879. (doi:10.1016/S0076-6879(07)37008-0) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:28099)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL: http://dx.doi.org/10.1016/S0076-6879(07)37008-0

Abstract

Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.

Item Type:	Article
DOI/Identification number:	10.1016/S0076-6879(07)37008-0
Subjects:	Q Science
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Depositing User:	Mark Shepherd
Date Deposited:	01 Sep 2011 15:57 UTC
Last Modified:	16 Nov 2021 10:06 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/28099 (The current URI for this page, for reference purposes)

University of Kent Author Information

Nicholls, Peter J..

Creator's ORCID:
CReDIT Contributor Roles:

Shepherd, Mark.

Creator's ORCID:	https://orcid.org/0000-0002-7472-2300
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